Lanarkshire MSPs Alex Neil and John Pentland together claimed expenses totalling more than £40,000 last year.
Mr Neil, MSP for Airdrie and Shotts, claimed £24,610 in total, while Mr Pentland, MSP for Motherwell and Wishaw, had expenses of £16,679 in 2011/12.
Included in these figures was a bill for £8627 worth of hotel stays in Edinburgh for Mr Neil, the Cabinet Secretary for Health and Wellbeing.
Travel was a another major expense, making up one-fifth of Mr Neil’s final bill at £4806 and contributing £3785 towards Mr Pentland’s total costs.
The figures show Mr Neil, who is the Cabinet Secretary for Health and Wellbeing spent £2828 more than his predecessor, Karen Whitefield, did in 2010-11.
He said: “It costs money to run an office, particularly when you get the level of correspondence and generate the amount of work I do.
“I am always trying to keep costs to a minimum but many are unavoidable.”
With reference to his costly hotel stays, Mr Neil said: “It is obviously important that when Parliament is sitting I am based in Edinburgh to attend to Parliamentary and Ministerial business.
“It is just not practical or feasible for me to commute to Parliament when most days I start at my desk at 7.30am and normally have meetings until eight or nine at night.”
Mr Pentland spent £2667 less than his predecessor Jack McConnell in the previous financial year 2010-2011.
Mr Pentland said: “ While I am and will continue to be careful with my allowances, I consider this is secondary to doing my job to the best of my ability.
“My claims are the costs incurred in order to represent my constituents in the constituency, to provide them with a service and to speak up on their behalf in the Scottish Parliament.”
The smallest claim made by the health secretary was 80p for car parking in November 2011while Mr Pentland documented a £1.56 television payment in January 2012.
Their largest respective single claims were for renting their constituency offices.
Mr Pentland also claimed back £2.54 for two packs of napkins for his office in November 2011 and claimed £4 for a refreshment with his evening meal.
In total, MSPs claimed back more than £12.8 million pounds in 2011/12, nearly £1million more than the previous year.
The total of £12,818,835 in 2011/12 is £969,436 higher than the £11,849,399 claimed by Holyrood’s politicians in 2010/11. It is due to winding-up expenses claimed by MSPs who either stood down or were not returned to the Scottish Parliament in 2011.